| A | B |
| Three Witches | Evil prophets that guide Macbeth's destiny with incomplete information regarding his future |
| Macbeth | Thane of Glamis |
| Lady Macbeth | Macbeth's wife and supporter of her husband's quest for power |
| Duncan | King of Scotland |
| Malcolm | Duncan's older son |
| Donalbain | Duncan's younger son |
| Banquo | General in the Scottish Army and Macbeth's friend |
| Fleance | Banquo's son who is seen as a threat by Macbeth |
| Macduff | Nobleman of Scotland and rival of Macbeth |
| Lady Macduff | Macduff's wife |
| Son | Macduff's son |
| Lennox and Ross | Noblemen of Scotland that support Malcolm's fight against Macbeth |
| Angus | Nobleman of Scotland and supporter against Macbeth |
| Menteith and Caithness | Noblemen of Scotland in Malcolm's English Army |
| Porter | servant at Macbeth's castle |
| Murderers | Macbeth's hired killers |
| Hecate | Goddess of the Witches |
| Apparitions | Visions conjured up by the Witches to inform Macbeth of what he should fear for the future |
| Doctor and Gentlewoman | Servants that witness Lady Macbeth's sleepwalking |
| Seyton | An Officer in Macbeth's Army |
| Siward | General in the English army fighting with Malcolm |
| Young Siward | Siward's son in the English army with Malcolm |
| Captain | Soldier in Duncan's military that reports on Macbeth's success in the battle against Macdonwald |