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RNA synthesis and processing, gene control

Extracted from pp. 299-332, 375-379 and 391-408 of Alberts et al.: Molecular Biology of the Cell, 4th ed. May contain errors - please comment. Updated 20/6 2004.

AB
snRNASmall nuclear RNA, functions in a variety of nuclear processes, including the splicing of pre-mRNA.
snoRNASmall nucleolar RNA, used to process and chemically modify rRNAs.
Sigma-factorDetachable subunit of bacterial RNA polymerase. Neccessary to initiate transcription.
RNA polymerase I-IIIEucaryotic polymerases. Require general transcription factors. I transcribes rRNA. II all protein coding genes plus some, III the rest.
TFIID / TBPA general transcription factor in eukaryotes. Contains the subunit TBP (TATA-binding protein).
CDTC-terminal domain of RNA polymerase II. Holds capping, splicing and polyadenylation factors once phosphorylated.
TATA boxConsensus sequence in promoter of many eucaryotic genes.
SupercoilLoop in DNA caused by superhelical tension.
DNA gyraseCreates negative supercoils to counter positive supercoils in bacteria.
LariatAn intron released during splicing. Shape of a lasso.
SpliceosomeAssembly of snRNA and proteins that perfom splicing in eucaryotes.
Gene activator and repressor proteinsGene regulatory proteins. Increase resp. inhibit transcription, former often by recruiting gen. transcr. factors and polymerase.
Lac OperonGroup of genes in E. coli transcribed as one mRNA. Under positive control of CAP and negative control of lac repressor.
Consensus sequenceMost typical sequence reproduced with minor variations in related DNA, RNA or protein sequences.


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