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Smear Making & Staining

These games are to aid in learning objectives 1-9 for Unit 2, Basic Hematology Tests.

AB
RomanowskyPolychrome Stain
PolychromeMany colors
PBSPeripheral Blood Smear
BMBone Marrow
Golgi ComplexClear Area Adjacent to Nucleus
ChromatinDark Purple
ParachromatinWhite or clear
ChromatinNucleus
ParachromatinNucleus
NucleolusNucleus
NucleolusPale Lavender
Endoplasmic ReticulumBlue Color to Cytoplasm
MembraneNot Visible
MitochondriaNot Visible
GiemsaPolychrome Stain
GiemsaRomanowsky Stain
WrightPolychrome Stain
WrightRomanowsky Stain
Buffer pH < 6.4RBC red orange
Buffer pH < 6.4WBC Nuclei lavender
Buffer pH < 6.4Platelets pale lavender
Buffer pH 6.4RBC red pink
Buffer pH 6.4WBC Nuclei purple
Buffer pH 6.4Platelets lavender
Buffer pH 6.4Neutrophil granules lilac lavender
Buffer pH < 6.4Neutrophil granules pale pink
Buffer pH > 6.4RBC bluish red
Buffer pH > 6.4Neutrophil granules dark lavender
Buffer pH > 6.4Eosinophilic granules pale pink
Buffer pH 6.4Eosinophilic granules red orange
Smear 2/3 to 3/4 length of slideCorrect
Smear < 1/3 length of slideCause: Pushed too fast
Smear < 1/3 length of slideCorrect: Push slower
Smear > 3/4 length of slideCause: Pushed too slow
Smear > 3/4 length of slideCorrect: Push faster
Smear > 3/4 length of slideCause: Angle too low
Smear > 3/4 length of slideCorrect: Increase angle
Smear > 3/4 length of slideCause: Drop too large
Smear > 3/4 length of slideCorrect: Smaller drop
Smear < 1/3 length of slideCause: Angle too sharp
Smear < 1/3 length of slideCorrect: Decrease angle
Smear < 1/3 length of slideCause: Drop too small
Smear < 1/3 length of slideCorrect: Increase drop
Acid ComponentsStain Blue with Methylene Blue
Basic ComponentsRed with Eosin
NucleiMethylene Blue
Eosinophilic GranulesEosin
RBCEosin
Neutrophilic granulesBoth Methylene Blue % Eosin
Basophilic granulesMethylene Blue
Eosinophilic granulesEosin
ProteinMethylene Blue


Robin Smith

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