A | B |
Color produced by longest ways of visible spectrum | Red |
Color that absorbs almost all the light it receives | Black |
Six main colors of visible spectrum | Violet, blue, green, yellow, orange, red |
Color produced by shortest rays of visible spectrum | Violet |
Wavelengths over 700 nm | Infared |
Wavelenths below 400 nm | Ultraviolet |
Holmium Oxide filters | Wavelength calibration |
Working standards | Standard curve |
Determine "a" in Beers law | Spectral absorbance curve |
Determine "a" in Beers Law | Spectral transmittance curve |
"a" in Beers Law | Absorptivity of solution |
"a" in Beers Law | Choosing best wavelength to read absorbance |
"b" in Beers Law | Path length of radiant energy |
"b" in Beers Law | Matched cuvettes |
"c" in Beers Law | Concentration of analyte in solution |
Photodetector | Photomultiplier Tube |
PMT | Photomultiplier Tube |
Stray Light | Misaligned source lamp |
Diffraction grating | Monochromator |
Prism | Monochromator |
Didymium Filter | Wavelength calibration |
Stray Light | Cut-off filters |
Neutral Density filters | Linearity checks |
Monochromator | Selects for wavelength |
Tungsten Lamp | 400 - 700 nm |
Deuterium Lamp | < 400 nm |
2 - log %T | Absorbance |
Linear graph paper | Abs vs. Conc |
Semi-log graph paper | %T vs. Conc |
Absorbance & Concentration | Directly related |
%T & Concentration | Inverse and Logarthmically related |