| A | B |
| Charlie Gordon | 37 year old mentally challenged man |
| Miss Kinnian | adult school teacher |
| Dr. Nemur | pessimist who wants to be famous in the psychology world and get the chair of psychology at Princeton |
| Dr. Strauus | optomist who cares about the emotional well being of Charlie |
| Frank Reilly | factory co-worker who made fun of Charlie |
| Joe Carp | factory co-worker who made fun of Charlie |
| Mr. Donnegan | boss of the plastic box factory |
| Amos Borg | foreman at the factory |
| Mrs. Flynn | landlady of Charlie's apartment |
| Burt Selden | laboratory employee who administered pschological tests to Charlie |
| Dishwasher boy | mirror image of Charlie's former self |
| Algernon | foreshadowing of Charlie; test mouse |