| A | B |
| Theseus | Duke of Athens |
| Hippolyta | Queen of the Amazons |
| Philostrate | master of ceremonies at Theseus' court |
| Egeus | father of Hermia |
| Hermia | loves Lysander |
| Helena | loves Demetrius |
| Demetrius | won Helena's heart, then abandoned her |
| Lysander | received a potion to make him love Helena |
| Helena | doubts Demetrius' professed love for her |
| Egeus | wants Athenian law enforced regarding his daughter |
| Titania | Queen of fairyland |
| Oberon | King of the fairies |
| Indian boy | son of Titania's mortal friend |
| Indian boy | subject of discord between Oberon and Titania |
| Puck | Robin Goodfellow |
| Puck | connecting element of all the plots |
| Peaseblossom | one of Titania's fairies |
| Mote | one of Titania's fairies |
| Mustardseed | one of Titania's fairies |
| Cobweb | one of Titania's fairies |
| Snug | the joinger; plays Lion |
| Flute | bellows mender; plays Thisbe |
| Snout | tinker; plays Wall |
| Starveling | the tailor; plays Moon |
| Peter Quince | the carpenter; director; delivers the Prologue |
| Nick Bottom | weaver; ass; Pyramus |