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Microbiology Test #1 (1)

Intro to Microbiology, History

AB
CDCCenter for Disease Control (Atlanta)
NIHNational Institute of Health (D.C.)
WHOWorld Health Organization
USPHSU.S. Public Health Service
Part of Cell TheoryAll living things are made of cells
Part of Cell TheoryCells are smallest living structures
Part of Cell TheoryAll cells come from preexisting cells
VirusesOnly reproduce and evolve, but only in another organism
Examples of microbes or microorganismsviruses, bacteria, protozoa, algae, fungi, invertebrate animals
Protozoa"first animals"
Pathogensdisease-causing organisms
Examples of Prokaryotesbacteria, cyanobacteria ("blue-green" algae)
Examples of Eukaryotesprotozoa, algae, fungi, plants, animals
Helminths"worms"
Micrometers (one millionth of a meter)what bacteria is usually measured in
Nanometers (one billionth of a meter)what viruses are usually measured in
Linnaeusdeveloped system of binomial nomenclature
binomial nomenclature"two-name naming system"
EubacteriaKingdom of unicellular prokaryotes
ArchaebacteriaKingdom of unicellular prokaryotes with unique cell walls
ProtistaKingdom of unicellular eukaryotes - ex. amoeba, paramecium, euglena, algae
FungiKingdom of multicellular, eukaryotic, nonmotile, heterotrophs
PlantaeKingdom of multicellular, eukaryotic, nonmotile, autotrophs
AnimaliaKingdom of multicellular, eukaryotic, motile at some point, heterotrophs
Abiogenesis"no life in beginning" "spontaneous generation"
AbiogenesisTheory believed 300 years ago that living things can come from nonliving matter
JanssensDeveloped first compound light microscope in late 1500's
MicroscopeFirst important advancement of microbiology
Galileo and KeplerInstrumental in making microscopes better
HookeIn 1600's, first to use the term "cell"
van LeeuwenhoekIn 1670's, "father of microbiology"
van LeeuwenhoekFirst scientist to see, describe, draw microorganisms
Pasteur (Late 1800's French)Disproved spontaneous generation with swan-neck flask experiment
Pasteur (Late 1800's French)"Pasteurization" saved French wine industry
Pasteur (Late 1800's French)Saved silk worm industry
Pasteur (Late 1800's French)Developed vaccines (rabies)
Pasteur (Late 1800's French)Worked on Germ Theory of Disease
Pasteur (Late 1800's French)First to describe "virus"
Koch (Late 1800's German)Proved Germ Theory of Disease
Germ Theory of DiseaseSpecific bacteria do cause specific diseases
Koch (Late 1800's German)His "postulates" showed you can identify which bacteria causes each infectious disease
Koch (Late 1800's German)Responsible for development of many of the pure culture techniques we use today
Koch (Late 1800's German)First to use agar
Agarused to grow bacteria
Bacillus anthracisBacteria that causes Anthrax
Koch (Late 1800's German)Developed techniques to isolate bacteria in pure culture
Koch (Late 1800's German)With Ehrlich, developed many stains used to see bacteria
Koch (Late 1800's German)One of his lab associates was named Petri
IvanovskiIn 1892, discovered Tobacco Mosaic Virus
Tobacco Mosaic VirusFirst virus discovered in 1892
Jenner (1700's)Developed first vaccine in the Western World
Jenner (1700's)Developed vaccine for small pox
Semmelweis (1800's)First instructed medical personnel to wash hands after investigating Puerperal fever
Lister (1800's)First to use disinfectants in surgery
Lister (1800's)Surgical death rate fell from 90% to 20% following his discovery
Chemotherapeutic agentsChemicals that treat disease
Ehrlich (early 1900's)Developed first synthetic drug to fight infection
SalvarsanFirst synthetic drug developed to fight infection (syphilis)
Treponema pallidumBacteria that causes syphilis
Three types of microscopesSimple light; compound light; electron
Simple light microscopeOne glass lens, often called magnifying glass
Simple light microscopeHas approximately 2-30x magnification
Compound light microscopeTwo or more glass lenses
Compound light microscopeMaximum magnification is 2000x
Compound light microscopeModern ones are parfocal
ParfocalOnce a specimen is focused using one objective lens, it is in relative focus for all objectives
Bright field compound light microscopeMost common type of compound light microscope
Phase contrast compound light microscopeEnables viewer to see better intracellular details in unstained cells (i.e. live cells)
Dark field compound light microscopedisc helps to see cells we cannot otherwise see; used to better see motility
Fluorescent compound light microscopeUsed to see things that fluoresce; used in some serologiccal diagnostic tests
Electron microscopeMicroscope developed in 1940's
Electron microscopeImages are called "electron micrographs"
Electron microscopeElectromagnets replace glass lenses; thin sectioning used; vacuum chamber
Electron microscopeSpecimens are already dead using this microscope
TEMAbbrev. for Transmission Electron Microscope
SEMAbbrev. for Scanning Electron Microscope
TEMHas 1,000,000x magnification
SEMHas 150,000x magnification
TEMUsed to see internal details of cells and organelles as well as viruses
SEMUsed to sese surfaces of specimens (3D-like image)


Linda

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