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Genetics: DNA Fingerprinting

Review of DNA fingerprinting

AB
fragments of DNA cut by restriction enzymes and unique to an individualRFLPs
process of separating DNA fragments via an electrical fieldelectrophoresis
process of transferring DNA embedde in a gel to a nylon sheet for further analysisSouther Blot
a seaweed extract; acts as a sieve in electrophoresisagarose
DNA designed to search out complimentary segments of DNA for location identification; usually radioactiveprobe
the process of using a radioactivity to expose photographic film for location identificationautoradiography
process of making many copies of small amounts of DNA by raising and lowering the temperaturePolymerase Chain Reaction
requirements of gene mapping1) multigenearational family; 2) probes to detect RFLPs 3) coordination between afflicted members of a pedigree & their bands on a Southern Blot test
examples of DNA sources from a crime sceneblood; skin cells; hair; semen


Chemistry Teacher
Pequea Valley High School

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