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Chapter 9 - "DNA: The Indispensible Forensic Science Tool"

Use these exercises to learn and review the basic terminology used to describe DNA and its forensic uses.

AB
amelogenin genegenetic locus useful for determining gender
amino acidsbuilding blocks of proteins
buccal cellscells from the inner cheek lining
chromosomethreadlike sructure in the cell nucleus along which genes are located
complementary base pairingpairing of A with T and G with C in DNA
deoxyribonucleic acidmolecules that carry genetic information
electrophoresisseparation of molecules through migration in a medium under electrical potential
epithelial cellsouter layer of skin cells
human genomeorder of bases on all 23 pairs of human chromosomes
hybridizationprocess of joining complimentary strands of DNA
low copy numberfewer than eighteen DNA-bearing cells
mitochondriacell organelle that provides energy for cells contains maternal DNA
multiplexingtechnique to simultaneously detect more than one DNA marker in a single analysis
nucleotidebuilding blocks of DNA molecule
picogramone trillionth of a gram
polymerlarge chemical substances composed of repeating units called monomers
polymerase chain reactiontechnique used for replicating DNA outside of a living cell
primershort DNA strand used to target a region of DNA for replication by PCR
proteinsamino acid polymers that play basic roles in the structure and function of cells
replicationsynthesis of new DNA from exisiting DNA
restriction enzymechemical that cuts DNA molecules open at specific locations
restriction fragment length polymorphismsdifferent fragment lengths of base pairs resulting from cutting a DNA molecule with a restriction enzyme
sequencingprocedure used to determine the base pairs in a piece of DNA
short tandem repeatregion of DNA that has short segments of 3 to 7 repeating base pairs
substrate controlunstained object adjacent to aan area with a deposite of biological material
tandem repeatchromosome region with multiple copies of a core DNA sequence
Y-STRsshort tandem repeats found on the human Y chromosome
DNA ligaseenzyme that joins pieces of DNA made by polymerases
RNA primaseenzyme that ays down RNA primers during DNA replication
SSBproteins that help unwind DNA
DNA plymerase IIIenzyme that builds DNA by adding complimentary nucleotides
DNA helicaseenzyme that opens and unwinds the DNA molecule
replication forkopened area of DNA helix where replication occurs
leading strandside of DNA where replication is faster because it is continuous
lagging strandside of DNA where replicaion is slower because it occurs in pieces
Okazaki fragmentspieces od DNA joined together on lagging side
Taq polymeraseenzyme derived from hot springs bacteria used in PCR reactions
pyrimidine basedouble ringed nitrogenous bases - adenine & guanine
purine basesingle ringed nitrogenus bases - cytosine, thymine, and uracil


Chemistry Teacher
Pequea Valley High School

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