| A | B |
|---|
| MICRON | 1/1000 of a mm or 0.001 of a mm |
| ROTARY MICROTOME | can adjust angle of knife inclination (clearance angle) |
| CLINICAL FREEZING MICROTOME | Ribbons not usually formed |
| CRYOSTAT | temperature is generally set at -20ºC |
| WATERBATHS | kept at 5-10 degrees below the melting point of the paraffin |
| INCUBATOR | temperature is usually kept at 37ºC |
| DRYING OVEN | routine temperature is between 50-60ºC |
| REFRIGERATOR | set at 4ºC |
| WEDGE KNIFE PROFILE | Most versatile type |
| PLANO CONCAVE KNIFE PROFILE | used in celloidin techniques |
| Clearance angle | 3-8 degrees |
| bevel angle | 27-32 degrees |
| Wedge angle | 15 degrees |
| Diamond knives | used for ultra thin sectioning in em |
| Glass knives | used for acrylic resins or plastics |
| Sections stick to anti-roll plate | plate is too warm |
| Sections are thick & thin (venetian blind) | improper knife angle or dull knife |
| Sections collect on knife-edge | temperature too warm, or anti-roll plate incorrectly positioned |
| Cryostat is stiff | clean and lubricate the microtome |
| Oven temp for CNS & Immuno | 37ºC |
