| A | B |
|---|
| Know how to plug numbers into the resolution equation (i.e. you don't need to know what lambda is equal to) | R=(0.61) wavelength/numerical aperture; wavelength = 0.54=lambda |
| wavelength | 0.54=lambda |
| R | (0.61) wavelength/numerical aperture |
| Know what enzymes are and what they do | Enzymes are proteins that make reactions occur more rapidly by lowerng the activation energy |
| Know the enzymes, substrates, and product in the reaction used in this lab | Substrate=Catechol; Enzyme=Catechol oxidase; Product=benzoquinone |
| Product in the reaction | benzoquinone |
| Enzyme in the reaction | Catechol oxidase |
| Substrate in the reaction | Catechol |
| Know how temperature and pH affect enzyme activity for the enzyme used in this lab | Higher temperature reduces activity; pH<4 or pH>8 reduces activity |
| Know how temperature and molecular weight affect the rate of diffusion | Higher temperature=increase rate of diffusion; Smaller molecues diffuse over a greater distance at faster rate |
| Diffusion | net movement of molecules "down a gradient" from high to low concentration |
| Osmosis | movement of water across a selectively permeable membrane from high to low concentration |
| Enzymes | are proteins that make reactions occur more rapidly by lowerng the activation energy |
| Higher temperature | increase rate of diffusion |
| Smaller molecues | diffuse over a greater distance at faster rate |
| In the dialysis bag experiment, the bags containing distilled water | lost weight when placed in a hypertonic solution with 15-30% NaCl |
| In the dialysis bag experiment, the bags containing 15-30% NaCl | gained weight when placed in a hypotonic solution with distilled water |
| Know why the salt solution plasmolyzed Elodea and what it looked like | The salt solution was hypertonic compared to the plant cells. The cells looked like the inside had shrunk, while the cell wall remain unchanged. |
| Yeast | undergoes fermentation when it is lacking O2. |
| CO2 | is a by product of fermentation. |
| Crickets and beans perform | respiration to make energy. |
| Crickets and beans | inhale O2 and produce CO2. |
