| A | B |
| Edward Armstrong | A successful London medical doctor |
| William Blore | A private detective and ex-Scotland Yard Inspector |
| Emily Brent | An older, self-righteous person who is fond of reading the Bible |
| Vera Claythorne | A young person who has come to Indian Island to be Mrs. Owen's secretary |
| John Macarthur | A retired general who seeks peace |
| Anthony (Tony) Marston | A reckless, God-like person who drives a flashy sports car |
| Mr. & Mrs. Rogers | The caretakers (cook and butler) on Indian Island |
| Justice Wargrave | A retired judge |
| Hugo Hamilton | Vera Claythorne's former lover |
| Fred Narracott | The Sticklehaven sailor who conveys the guests and supplies to Indian Island |
| Isaac Morris | Person used by U. N. Owen to purchase Indian Island |
| U. N. Owen | The mysterious “owner” of Indian Island, who invites most of the guests there |
| Philip Lombard | A former member of the military who carries a gun |