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Staining II ; Unit 3 Part I

AB
Glycogen & MucinMost commonly found Carbohydrates in the body
SugarsMonosaccharides, disaccharides, polysaccharides
Bauer-Fuelgendemonstrates glycogen
Bests Carminedemonstrates glycogen
Periodic AcidOxidizer used in PAS method
PAS/DiastaseDemos glycogen
RossmanGlycogens fixative of choice
Starch, Cellulose, ChitinCarbohydrates that do not require fixation
Periodic Acid is preferred becase?It stops at the aldehyde stage
Transient colorless unstable dialdehyde compoundResult from exposure of dialdehydes to Schiffs reagent
Sulfurous RinseRemoves excess schiff, prevents false pigmentation from oxidation during coverslipping
Neutral mucinsFound in basement membrane, stomach, thyroid
non sulfated sialomucinsgoblet cells, salivary glands, pancreas


Goodwin College
East Hartford, CT

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