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Staining II ; Unit 5

AB
What gives Alcian Blue its blue colorCopper in the molecule
Digestion with Hyaluronidase involvesDepolymerization of the substrate, hyaluronic acid, causing loss if basophilia
Three types of HyaluronidaseBacterical, testicular, Chondroitinases
Results of low Iron DiamineAll sulfated and many nonsulfated mucins = black, some unsulfated = blue
Results of high Iron DiamineSulfated mucins = gray-purple-black, nonsulfated = unstained
Carbohydrate which is not PAS positiveHyaluronic Acid
Stains positive with aldehyde fuchsinHyaluronic Acid
What is added to Crystal Violet to prevent heavy orthochromatic stainingAcid
Amyloids characteristics of microtomycut at 10 micrometers, beta pleated sheets, control tissue should not be stored for long time
What methods demonstrate AL AmyloidIodine, Puchtlers mod. of Congo Red, Congo Red
Methods that demonstrate AA AmyloidHighmans methyl violet, Leib's crystal violet, Crystal Violet
Define Negri bodiesrabies
Methods that demonstrate negri bodiesParsons, Schleifsteins
Fixative for ParonsFormaln
Fixative for SchleifsteinsZenkers
Individual viruses are visible under what type of microscopeEM
GMS demonstrates whatPneumocystis carinii
Methods that demonstrate Hepatitis B Surface AnitgenAldehyde fuchsin, Orcein
This image shows Alcian Blue with?, Hyaluronidase


Goodwin College
East Hartford, CT

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