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Staining II ; Unit 3 Part II

AB
Function of mucinslubrication, coat cell surfaces, provide adhesion
Strongly sulfated acidic mucins (C.T)Keratin sulfate,heparin,heparin sulfate, dermatin sulfate
Two types of Nonsulfated Acidic MucinsSialomucins, Hyaluronic Acid
Alcian blue pH 0.5 stainsStrongly sulfated(C.T), Mast cells
Mucicarmine stainsNonsulfated sialomucins
Metachromatic Dyes pH 2.0 stainsStrongly sulfated(C.T), weakly sulfated sulfomucins
Colloidal Iron stainsStrongly sulfated(C.T such as heparin sulfate)
Two Metachromatic Dyes which demonstrate Mast cellsAlcian Blue, Aldehyde Fuchsin
Which dyes stain Weakly Sulfated Goblet CellsPAS, Alcian Blue pH 2.5, Metachromatic pH 2.0, Colloidal Iron
Neutral Mucins are stained byPAS
Nonsulfated Sialomucins in the Epithelium are NOT stained byAlcian Blue pH 0.5, Metachromatic pH 2.0
Three types of sugarsMonosaccharides, disaccharides, polysaccharides
Tests that demonstrate homoglycansPAS, PAS/DIASTASE, Best Carmine, Bauer-Feulgen
Glycogens fixative of choiceRossmans
Carbohydrates that do not require fixationStarch, Cellulose, Chitin
This section of small intestine can also be stained by?, Mucicarmine
What is staining pink?, Goblet cells
What type of stain and tissue is this?, Alcian Blue - Umbilical Cord
What is staining brown-black, Nuclei


Goodwin College
East Hartford, CT

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