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Staining II ; Unit 4 Glycogen

AB
Results of Bauer-Feulgen Reaction?Glycogen, Fungus, Certain mucins - Red/purple, Nuclei - Blue
Lillies Azure A-Eosin B Method stains?Paneth cells granules - Pink to red
Bauer Fuelgens oxidizerChromic Acid
Enzymes used to digest out glycogenAmylase, Diastase, Saliva
Technique specific for glycogenPAS with and without Diastase
Which fixatives will preserve PanethCells in the small Intestine and Zymogen cells in the pancreasNBF, mercuric chloride formalin, orth, kose, Mollers dichromate formalin
Staining method which demonstrates paneth granulesLillies Azure A - Eosin B method
Staining method which demonstrates pancreatic alpha and beta cellsCombined Gomori Methods
Staining method which demonstrates alpha,beta and delta cells of pancreasTrichrome- PAS
Results of the Best's Carmine methodAmoeba- Red, glycogen, fibrin, neutral mucin - pink red, Nuclei - blue
Neutral mucins can be distinguished from Acidic mucins byMetachromatic dyes, mucicarmine, hydrazine, colloidal iron, alcian blue
The mucicarmine method especially demonstratesEpithelial mucins
Definition of anhydrouswithout water
What forms a celate compound with the carmine in the Mucicarmine techniqueAluminum Salts
Which type of hematoxylin is used in the Mucicarmine methodWeigerts
What is the differing ingredient between Mayers Mucicarmine and Southgates Mucicarminaluminum chloride & aluminum hydroxide
Two metachromatic dyes used for demonstrating mucinsToludine Blue, Methylene blue
Define the term saponificationTo convert
Ingredients of Perls ReagentPottasium Ferrocyanide, HCl


Goodwin College
East Hartford, CT

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