| A | B |
|---|
| Crystal Violet is used in the Gram staining method to stain | Gram positive & negative bacteria blue/black |
| The mordant is used in the Grams method for? | distinguishing between positive and negative bacteria |
| Characteristics of Gram Positive bacteria | More lipoprotetin then negative cells, more polysaccharides, has magnesium ribonucleate, cell walls are 3-4 layers |
| Picric Acetones function in Taylors Mod. Brown-Brenn stain is? | to decolorize background to brownish yellow |
| Methods which demonstrate Listeria Monocytogenes | Gram & Steiner |
| Property of Acid Fastness | The lipid capsule makes the organism resistant to alcohol or acid treatment after staining |
| Results of Kinyouns Acid Fast stain | AFB= bright red, Background = blue |
| What is the dye used in Zeihel Neelsens Carbol Fuchsin solution | Basic Fuchsin |
| Method that demonstrates nonviable acid fast bacilli | Auramine-Rhodamine, Truants |
| Which step in the Norcarid Procedure is timing critical | Carbol Fuchsin |
| Another procedure to stain AFB | Wades Mod of Fites New Fuchsin Formaldehyde |
| Fluorescent method used to demonstrate AFB | Auramine-Rhodamine |
| Why is paraffin removed with turpentine,xylene, and peanut oil in Wades Mod of Fites New Fuchsin Formaldehyde | Helps remedy the harmful effects of tissue processing |
| Spirochetes usually require | silver impregnation |
| Define argyophilic | Absorbs silver salts but needs to be reduced |
| Characteristics of Chlamydia and Rickettsia | Obligate, Intracelluar parasites, contains DNA and RNA |
| Precaution used in fungus staining | Use mold inhibitors to prevent fungus contaminating the reagents |
| Oxidizer used in GMS | Chromic Acid |
| Why is Chromic Acid used in GMS | It is a strong oxidant that oxidizes polysaccharides in fungal walls to aldehydes |
| Methods that demonstrate Rickettsias | Wright, Pinkerton, Giemsa |
