| A | B |
|---|
| Lipids are soluble in? | Organic solvents |
| What is the best method to demonstrate lipids | Frozen |
| What are the types of unconjugated lipids | fatty acid, cholesterol |
| What are the types of conjugated lipids? | ester lipids, phospholipids |
| Name the conjugated lipids which are hydrophobic | Cholesterol esters, mono ditriglycerides, waxes |
| Define hydrophilic | Likes water |
| What lipids are hydrophilic | phospholipids, acidic lycolipids, neutral glycolipids |
| Lipids in crystalline form are? | Isotropic and birefringent |
| Why isnt Primary Flourescence used as a diagnostic tool for lipids | Because only certain lipids procduce 1 fluorescence |
| Melting points, molecular weight of dye, and solubility of dye affects what? | The stainability of lipids with oil soluble dyes |
| Hot chloroform does what? | Removes all lipids |
| What solvents do not dissolve out lipids | isopropanol and propylene glycol |
| Why must you use precaution when using aqueous mountants for coverslipping | If you press on coverslip too hard to remove air bubbles, it may dislodge fat |
| What type of mounting media should be used with fat stains | synthetic resin |
| Phosphate 3R & 3.4 Benzpyrene are? | two secondary flourescent methods used to detect lipids in formalin fixed frozen sections |
| Permanent secondary flourescent method | Phosphate 3R |
| What two oxidizing agents are used in the Schiffs reaction for unsaturated lipids | Performic acid and Peractic acid |
| acidic and neutral lipids can be distinguished by? | Nile Blue sulfate |
| Technique that distinguishes between hydrophobic and hydrophilic lipids | OTAN |
| Fixative used in Bakers Acid Hematein method | Formal Calcium |
| Differentiator in Bakers Acid Hematein method | Borax ferricyanide |
| Hazards of Osmium textroxide? | Causes toxic vapors, may fix corneas |
