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Staining II ; Unit 7 Part 2

AB
What are the two functions of neurogliaprotects and insulates neurons, produces myelin sheath and regulates neuronal enviroment
What are the four main types of glial cellsastrocytes, oligodenroglia, microglia, ependymal
Two types of astrocytesprotoplasmic and fibrous
Oligodendroglia isthe glial cell that forms myelin
What is the function of astrocytesTo provide support, nourishment, and metabolic exchange
Microglia isthe glial cell that is a fixed phagocyte
What is the glial cell that is the most numerous and is located in the gray and white matterOligodendroglia
Ependymal isthe glial cell that is simple columnar epithelium
cresyl echt violet stains astrocytes whatpale blue
cresyl echt ciolet stains oligodendroglia whatvery dark blue
What does the trichrome stain demo?astrocytes, nuclei, nerve cells
Cajals method stains?astrocytes deep purple
Holzer demonsrates?Glial Fibers blue
What methods demonstrates myelin?Weil, Pal Weigert, Marchi, LFB
Marchi demonstrates?degenerating myelin - black
LFB stains?Myelin - blue/green, cells - pink/violet
LFB can be combined with what other staining methodsPAS, PTAH, Oil red O, Holmes
Unlike Alcian blue, LFB is?Alcohol soluble
What is Myelin composed ofProtein, cholesterol, phospholipids, cerebrosides
What does the Marchi method use to stain degenerating myelinosmium textroxide


Goodwin College
East Hartford, CT

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