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Staining II ; unit 9 Part I & II

AB
Definition of artifactDeposits artifactually produced by chemicals
Define EndogenousProduced by tissue within tissues, serve a physiological function
Define ExogenousGain access into the body accidently, serve no physiological function
Characteristics of LipofuchsinIncrease with age, Iron negative gives brown flourescent with UV light, resistant to alcohols and paraffin processing
Characteristics of CeroidBrown acid fast pigment, insoluble in alcohols, negative prussian blue reaction
Pheochromocytomas arechromaffin cell tumors of the adrenal gland
The Grimeluis staining method demonstratescarcinoid tumors and alpha cells of the pancreas
Anthrocotic pigment isblack carbon commonly in lymph nodes and lungs
Oxalosis isautosomal recessive disease, oxalate deposit found the heart, kidney, bone, testes, and blood vessel
What type of fixation should you use when using the schmorl reduction methodA Chromate fixative
What are the two substances that are responsible for chromaffin reaction5-hydroxtyptamine and catecholamine
What are some methods that demonstrate argentaffin cells?Fontana masson, schmorl reducing, Gomori Burtner, Diazo, autofloursecent
What are three types of APUD cellsstomach, duodenum & small intestine, kulchitsky cells
Why must alcohol containing fixative be avoided?It dissolves argentaffin granules
What method is positive for carcinoid tumors of the appendix and small intestine and negative for bronchial carcinoids?Diazo
What are the bleaching solutions for melaninPotassium Permanganate, hydrogen peroxide, performic acid and peracetic acid
What staining methods demonstrate melanin pigment?Gomoris Meth silver, fontana masson, ferrous iron uptake, schmorls, DOPA oxidase
Substantia nigra is?The portion of the brain where melanin is found
What is gouty TophiUrate deposits around joints, a uric acid metabolism disorder
Bodian stains?Carcinoid granules brown/black


Goodwin College
East Hartford, CT

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