| A | B |
|---|
| What effect may fixation have on Ag binding sites? | It can mask the sites |
| Disadvantage to using fresh tissue? | Enzymes are displaced from original site in tissue |
| What is the most rapid freezing method | Liquid nitrogen and prechilled isopentane method |
| In Method A of fixed frozen sections, what fixatives can be used? | Cold Formalin or cold formal calcium |
| What fixatives are used in Method B? | Cold formalin, formal calcium or acetone |
| How thick should sections be cut on the cryostat for the ATPase Isopentane method | 8-10 micrometers |
| What type of reactions may be useful in determining tumor origins and progression of cervical cancer? | Esterase reactions |
| What are two types of esterase reactions | Fast blue RR and Leder |
| Which esterase method gives higer resolution and distinguishes granulocytic from lymphocytic leukemia | Leder |
| What is used for vascular endothelial tumor? | Alkaline phosphates |
| What type of Ab is HMB-45? | monoclonal |
| What is usedto demonstrate neuritic plaques in Alzheimers? | B Amyloid |
| What does GFAP demo? | astrocytes |
| What type of solution is DOPA used in | buffered |
| DOPA demos? | Melanin forming cells |
| What is glucose oxidase and horse radish peroxidase? | 2 most widely used enzyme labels |
