| A | B |
|---|
| Routine specimen section thickness should be? | 4-6 microns |
| Amyloid section thickness should be? | 10 microns |
| Myelin section thickness should be? | 10-15 microns |
| Small biopsies section thickness should be? | 2-4 microns |
| Examples of soft tissue | brain, spinal cord, lymph node |
| What is a problem caused by soft tissue | compression & stress lines |
| What are some causes for difficult tissue such as hard, bloody or tough | prolonged fixation, prolonged clearing, prolonged parffin infiltration, drying out of tissue |
| Examples of difficult tissue types | thyroid, cirrhosis, spleen, blood clots |
| What is a method for softening tissue | Preece & Lillie |
| What substances does Preece use | Detergent & water |
| What substances does Lillie use for imcompletley dehydrated tissue | Glycerol - aniline |
| Which biopsies are cut at 2 microns | Kidney |
| What is an example of a special stain requiring sections to be cut at 8 to 10 microns | Congo Red for amyloid |
| What is a serial section | Every section saved, the slides are numbered consecutivley |
| What are step sections | When lesions are not obvious, cut through the entire block taking levels |
