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Histology techniques Unit 9

AB
What is a pippette more accurate than a graduated cylinder when measuring small amounts?Its smaller
What type of solution would give you a fine black precipitateA silver nitrate solution
How do you get rid of the percipitate?Rinse with oxalic acid
Potassium dichromate, distilled water, and concentrated sulfuric acid are ingredients in?acid cleaning solution
Does stock or working solution have a longer shelf life?Stock solution
How would you remedy the problem of not using the proper fixative for the staining method?Mordant the tissue with zenkers or bouins
When formalin is used as a fixative how would it affect the H&E stain?formalin helps the nuclei become more basophilic
What solutions can you use to restain slides?potassium permanganate & oxalic acid
What solutions do you use to remove acid and basic dyes0.5% acid alcohol or 1% alcohol
How do you remove the Iron Lake5% Sodium Dithionite
What is the fixative of choice for PAPAlcohol or Methanol
What is the fixative of choice for Modified GeimsaZenkers
WHat is the purpose of using control slides?see if stain is staining properly, check that reagent is still active, how much nonspecific staining is occuring
WHat tissue is used to show elastic fibers?aorta, kidney, skin
What tissue is used to show mast cells?skin
what tissue is used to show glycogenliver
what tissue is used to show fatsadrenal cortex, fatty liver
what tissue is used to show mucinthe G.I tract


Goodwin College
East Hartford, CT

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