| A | B |
|---|
| This is a disadvantage of using heat on a tissue processor | Hardens tissues such as spleen, muscle, skin |
| What features are not an option on open tissue processors? | Heat & vents |
| How man mm above the cassettes does the fluid level need to be on the tissue processors | 13 |
| What is the allowed maximum thhickness of tissue | 3-4 mm |
| If tissue dries out while in the air on an open tissue processor, what would you do? | Place the tissue in sodium carbonate, d. H2O, absolute alcohol, and reprocess |
| What part of the processor removes air bubbles, removes clearing agent more rapidly, decreases impregnation time? | Vacuum |
| Rinsing in 95% alcohol, absolute alcohol, xylene and impregnating with paraffin on vaccuum remedies what? | If tissue goes into fixative after the paraffin |
| Why is it important to frequently change the solutions on the tissue processors? | to prevent build up of clearing agent in paraffin, and so alcohols dont get diluted |
| How often should the solutions be changed on the tissue processors? | depends on the number and size of tissue being processed |
| what is a good clearing solution which is good for uterus | use cedarwood oil |
| what solution is helpful for processing fat? | acetone |
| the volume of fluid should exceed that of tissue by? | 15 to 20 volumes |
| 4 main steps of tissue processing | Fixation/dehydration/clearing/infiltration |
| Dehydration | Removal of aqueous fluids in the tissue |
| Insufficient dehydration causes | Unsatisfactory clearing & infiltration |
