| A | B |
|---|
| What is the most important step in the process of producing a high quality microscopic tissue slide? | Good fixation |
| Digestion of tissue/ cells by enzymes normally present in the cells is? | Autolysis |
| Decay or breakdown of tissue by bacteria is? | Putrefaction |
| What are the two physical fixation agents? | Desiccation and heat |
| What is the cheif aim of a fixative? | To coagulate or precipitate protoplasmic substances such as protein in order to preserve microanatomical arrangement of tissue elements, and to stop autolysis, putrefaction |
| what is best for storing fixed tissue? | 70% alcohol |
| What are a few functions of a good fixative | rapidly penetrates, kills, hardens, renders tissue susceptible to appropiate staining, preserve tissue constituent of interest |
| What solution removes mercuric chloride pigment through an oxidization process? | Iodine & Hypo |
| Which fixative interferes with many staining methods and makes counterstaining difficult? | Osmium Tetroxide |
| Mercuric Chloride is? | a fixative & a mordant |
| How many volumes of fixative should be used for every volume of tissue | 15-20 |
| Why does tissue fixed in formalin and embedded in paraffin shrinks by 33%? | The volume changes during fixation |
| Which type of osmolal solution produces the best fixatio results? | slightly hypertonic |
| How do lower temperatures affect fixaton? | slows/delays fixation time, slows diffusion, slows autolysis |
| Fixative of choice for red blood cells, bone marrow, kidney, intercalated discs is? | Hellys |
| Fixative of choice for lymph nodes? | B-5 |
