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Basic Staining & Fixation Unit 2

AB
What prevents the formation of formalin pigment?Buffer
What pH should the buffer be to precent formalin pigment7
What does PEL stand forPermissible exposure limit
What is the PEL of formaldehyde for over 8 hours.75 ppm
What is STELShort term exposure limit
What is the STEL of formaldehyde for any 15 minute period2.0 ppm
What is acidophilicParts of tissue that are basic; they like acid dyes
What picric acid fixatives decalcify as they fixPFF and Hollande solution
When do you get a formation of chromic oxideWhen using potassium dichromate as a fixative
How do you prevent the formation of chromic acidby washing thoroughly with water
What component attaches to some lipids rendering them insoluble in lipid solventsChromium
HCHO is the chemical formula forformaldehyde
CHO CHO is the chemical formula forGlutaraldehyde
Formaldehyde is an excellent fixative forLipids during frozen sectioning
10% formalin is composed of?1 volume formalin; 9 volume H2O
What fixative's mechanism of action is crosslinking with proteinsFormalin
What are the fixatives of choice when preserving phospholipidsBakers Calcium, formalin calcium
Zamboni PAF isthe electron microscopy fixative that is stable at room temperature
ReductionLoss of oxygen
fixative of choice for PheochromocytomasOrth
K2Cr2O7 is the chemical formula forPotassium Dichromate
Formalin isexcellent for lipids during frozen sectioning
Purpose of using NBF vs. unbuffered formalinpurpose of using neutral buffered formalin vs. unbuffered formalin


Goodwin College
East Hartford, CT

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