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Basic Staining & Fixation Unit 3

AB
What are recommended fixatives for blood smearsAbsolute ethanol
What is a recommended fixative for rush biopsiesMethanol
What can be preserved by using absolute alcohol and methanol for fixationMast cells, Iron and copper
What fixative is used for fat demo, perservation of acid mucopolysacchrides, diagnosis of rabiesAceton
What is acetone not recommended forpreserving glycogen and certain lipids
What are the disadvantages of Carnoys fixativeSwells tissue, no acid fast stains, destroys rbcs, destroys paneth cells
What fixative is considered poor when demonstrating cytoplasmic componentsAcetic acid
What are the advantages of acetic acidcoagulates nucleic acid, renders chromatin basophilic, counteracts shrinking effects of alcohol on tissue
What fixative is not recommended as a lipid fixativealcohol
What is the isoelectic pointWhen positive and negative charges are balanced


Goodwin College
East Hartford, CT

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