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Basic Staining & Fixation Unit 4

AB
What are some methods for formalin pigment removalSaturated alcohol solution of Picric acid, ammonium hydroxide in 70% alcohol, Bleach by nitiric acid
Iodine and Hypo remove whatMercury pigment
Several changes of 50% alcohol and 70% alcohol for long periods of time removes whatPicric acid color
Washing tissue in water after fixation prevents whatPigment artifacts
What is a dark brown crystalline bifringence artifact caused byformalin in a blood rich tissue at pH levels below 5.6
how many volumes of fixative should be used for every volume of tissue15-20
Potassium dichromate, mercuric chloride, acetic acid, and sodium sulfate are ingredients in?Zenkers
Mercuric chloride, pottasium dichromate, sodium sulfate are ingredients in?Hellys
What are the ingredients in Carnoys?absolute alcohol, acetic acid, chloroform
Zambonis PAF contains?Paraformaldehyde, double filtered saturated picric acid
Which fixative contains copper acetateHollandes
What are the recommended fixatives for Lymph Nodes?B-5
What are the recommended fixatives for lipids and phospholipidsFormol calcium
What are the recommended fixatives for massons trichromeBouins
What are the recommended fixatives for glycogenRossmans
What are the recommended fixatives for light and electron microscopyParaformaldehyde


Goodwin College
East Hartford, CT

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