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Biology-Chapter 12

AB
genetic engineeringthe engineering of a set of genetic changes direcly into an organism's DNA
restriction enzymesproteins that can cut genes at specific DNA sequences
75Number of restriction enzymes known
each restriction enzyme recognizes a sight...4-6 nucleotide pairs long
Restriction enzymes make it possible to cut DNA into fragments that can be...isolated, sparated, and analyzed
DNA fragments must becomepart of the genetic material before they can be activated
In the 2nd step of genetic engineering...DNA fragments are incorporated into part of the recipient cell's genetic material
Plasmidssmall circular DNA molecules found in bacteria cells in addition to their chromosones
Plasmidscan be cut using the same restriction enzymes used to produce DNA fragments
cuts made by restriction enzymes on the DNA fragments and the plastidssticky cuts
sights where the DNA fragments and the plastids can be joined forming a new plastid containing a piece of foreign DNAsticky ends
the combined DNA consists ofparts from different kinds of organisms
In genetic engineering, molecules of combined DNA are known aschimeras becuase they are produces by combining DNA from different species
combined DNA is also known asrecombinant DNA because DNA from two sources have been recombined to produce it
it is easiest to transfer DNA intobacteria cells
the recombinant DNA is mixed withthousands of bacteria suspened in a dense salt solution
The term for a large number of cells grown from a single cell isclone
Using different techniques, recombinant DNAcan be inserted into cells other than bacteria-like yeast, animal, and plant cells
Injection with a glass needle is known asmicroinjection
The number of strands used in the process of DNA sequencing1
reading a piece of DNA is also known asDNA sequencing
multiple copies of a single strand of DNA can be optained byDNA cloning
DNA pieces are separated by a process calledelectrophoresis
organisms that contain foreign genes are said to betransgenic
protein that blocks the growth of virusesinterferon
Scientist that studied whether genes in animals can be made to work in plantsSteven Howell
enzyme that makes fireflies glowluciferase
production of genetically identical organisms from single cellscloning
the hepatits B vaccine was produced bygenetically engineering bacteria
all of the genes possesed by humansgenome
Fragments that contain repeats in DNA fingerprinting are labeled withDNA probe
DNA samples can be obtained fromblood, sperm, and hair strands with small pieces of tissue at the base
selecting a few individuals to serve as parents for the next generationselective breeding
individuals produced by hybridization that are hardier are known ashybrid vigor


Lee Mooney

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