| A | B |
| Charlie Gordon | undergoes an experimental operation |
| Alice Kinnian | teacher at Beckman special school |
| Fay Lillman | neighbor who's an artist |
| Rose Gordon | Charlie's mother |
| Matt Gordon | Charlie's father |
| Harold Nemur & Jay Strauss | neuro-surgeons and psychiatrists |
| Norma Gordon | Charlie's younger sister |
| Algernon | white mouse used in experiental operation |
| protagonist | central figure in a story; hero or heroine |
| antagonist | a character that opposes the hero/heroine; villiain |