| A | B |
|---|
| cDNA Library | a collection of genes made from RNA using reverse transcriptase and inserted into cloning vectors |
| ECoRI | an enzyme that can cut DNA at a certain sequence of nucleotides |
| Reverse Transcriptase | an enzyme used to convert RNA code into DNA code |
| PCR | A method of amplifying DNA using primers and a cycling of temperatures |
| Gel Electrophoresis | A method of sorting DNA fragments by length |
| Flourescent Dye Probe | Used to label specific genetic sequences and make them visible under special light (UV) |
| RNA Primer | A short segment of nucleotides that provides a starting point for polymerase |
| Plasmid vector | A small loop of DNA that can "carry" an inserted gene into a bacterial cell |
| Virus Vector | An infectious particle that can carry a gene into a bacterial cell |
| Ampicillin resistance gene | A gene that gives a bacterium the ability to grow in teh presence of an antibiotic |
| ddA,ddT,ddC and ddG | special nucleotides that shut down replication of DNA when added to the sequence |
| E coli | A bacterium used to express recombinant genes |
| Cycler tank | A machine used to heat/cool/heat/cool DNA |
| electroporation | A method of opening up the cell membrane of a eukaryotic cell temporarily |
| RFLP Analysis | A technique used to compare "DNA fingerprints" |
| Radioactive Probe | Used to label specific genetic sequences and make them show up on photographic paper |
