| A | B |
|---|
| Type of dye that stain the nucleus | Basic dyes |
| A greater concentration of hydroxyl ions gives | A higher pH or basic dyes (+) |
| Example of a Cationic dye | Hematoxylin |
| Metachromasia | Color shift |
| Amphoteric | An acid OR a base |
| Adsorption | Dyes adhere to surface of structure |
| Absorption | Tissue soak up dye & is penetrated by the dye |
| Causes of orthochromasia | Overstaining |
| Example of a Metachromatic dye | Bismarck brown |
| Fixatives of choice for metachromatic stains | Alcohol and Formalin |
| Basic dyes are attracted by | (-) charged macromolecules |
| Metachromic tissue substance | Mast cells |
| Factor that influence metachromasia | pH |
| Cause of false metachromasia | Fixatives with strong oxidants - K dichromate |
| Acidic staining sltn (pH 3-4) | Reduces orthochromatic effect |
| Coulombic attractions | Oppositely charged particles attract each other |
| Isoelectric point (IEP) | State of equilibrium, net charge of 0 |
