| A | B |
|---|
| Purpose of changing the alcohols on the tissue processors | To limit absorb water or moisture |
| Incomplete or unsatisfactory clearing & infiltration | Tissue not left in dehydrats long enough |
| Fixatives increasing basophilia | formalin & osmium tetroxide |
| Fixatives increasing acidophilia | mercuric chloride & acidic dichromate |
| Buffers | Maintain the pH of a solution |
| Fixation Factor - Agitation | Ensures fluids completely surround tissue on all sides |
| Fixation Factor - Heat | Increase rate of fluid penetration & tissue shrinkage |
| Fixation Factor - Ultrasonic | Not routinely used - increases rate of tissue processing |
| Fixation Factor - Viscosity | Fluid thickness, affects rate of tisse penetration |
| Fixation Factor - Vacuum | Remove air bubbles trapped in tissue |
| Correct volume ratio of tissue to fixative | 15 to 20 |
