| A | B |
|---|
| Ethanol’s disadvantages | Expensive and shrinks |
| Butanol’s disadvantages | slow, excessive hardening, shrinks |
| Dioxane’s disadvantages | Costly & cumulatively toxic |
| Acetone’s disadvantages | Needs good ventilation, flammable, shrinks, over hardens |
| Description of a universal solvent | Fixative, dehydrant & solvent |
| Examples of universal solvents | Tetrahydrofuran, Dioxane, Tertiary butanol |
| Fixative of choice for uric acid crystals | Absolute alcohol |
| Fixative of choice for enzymes, rabies & phospholipids | Acetone |
| Acetone | Not recommended for glycogen, certain lipids |
| Disadvantages of Carnoy’s | Renders AFB non acid fast, dissolves cell granules & pigments |
| Poor fixative to demonstrate cytoplasmic components | Acetic acid |
| Advantages of acetic acid | Counteracts shrinkage of alcohol |
| Chemical formula for Ethyl alcohol | C2H5OH |
| Chemical formula for Acetone | CH3COCH3 |
| Chemical formula for Acetic acid | CH3COOH |
| Fixative not recommend for lipids | Alcohol |
| Isoelectric point | Positive and negative charges being equal in number |
| Unsatisfactory clearing & infiltration | Water is left in tissue |
