| A | B |
|---|
| Incomplete deparaffinization | Uneven staining or unstained patches due to trapped water |
| When you see white patches in tissue during deparaffinization | Remove water, place slides in 100% alcohol, then back into xylene |
| Hydration | Brings water into tissue, prepares tissue for staining |
| Hydrations reagent of choice | Series of graded alcohols |
| Formalin pigment removal | Saturated alcoholic picric acid |
| Mercury pigment removal | Iodine and Hypo |
| Picric acid color removal | Series of 50% alcohol and 70% alcohol |
| Function of 95% alcohol after eosin | Differentiates & dehydrates |
| Hazy with poor nuclei contrast | Improper differentiation |
| Tissue is opaque | Incomplete dehydration |
| Milky white xylene | Contaminated with water |
| Prevention of chrome artifact | Washing the tissue in water after fixation |
| Chrome fixatives | Zenkers & Hellys |
| Dark brown crystalline birefringence artifact is caused by | Formalin at pH 5.6 |
| 15 to 20 | Volumes of fixative to volume of tissue |
