| A | B |
|---|
| Hematoxylin’s active coloring agent | hematein |
| Hematein is produced by | oxidation |
| Attaches dye to tissue | mordant |
| Oxidation | loss of hydrogen, gain of oxygen |
| oxidation occurs through | natural or chemical ripening |
| Delafield | naturally ripened hematoxylin |
| Sodium iodate | chemical ripening agent of Mayer/Gills |
| Mercuric oxide | chemical ripening agent of Harris |
| Affecting the rate of oxidation | amount used & pH |
| Filtering hematoxylin | remove precipitate caused by oxidation |
| Primary function of a mordant | gives dye affinity for tissue |
| Mordant used in Mayers & Harris | aluminum |
| Mordant used in Weigerts & Heidenhains | Iron |
| Tungsten & Lead | Metal mordants used in Hematoxylins |
| Metal mordant with a vigorous oxidizing action | Ferric Chloride |
| Weigert’s iron hematoxylin | short self life |
| Combination of mordant and dye | dye lake |
| Malignant nuclei | increase size, irregular nuclear outline |
| Heterochromatin or condensed chromatin | stains with basic dyes |
| Attraction b/w cationic dyes & anionic phosphoryl groups of DNA | electrostatic bonds |
| Nuclear protein bound to phosphoric acid residues of DNA | histone |
| Turn the reddish color of hematoxylin to blue | bluing |
| Ingredient in hematoxylin that causes it to become reddish | old alum |
| Preservatives for hematoxylin | chloral hydrate & alcohol |
| increases selectivity of the stain for nuclei | aluminum salts |
| Hematoxylins used regressively | Harris & Gill |
| Naturally ripening hematoxylin takes | 3 to 6 months with light & air |
