| A | B |
|---|
| Incomplete deparaffinization | Resulting in uneven staining or unstained patches |
| What should you do when you see white patches in tissue during deparaffinization | Place slides in 100% alcohol-then back into xylene |
| The function of hydration: | Brings H2O into tissue |
| Reagent of choice for hydration: | Series of grade alcohols |
| Method for formalin pigment removal: | Saturated alcoholic picric acid |
| Method to remove mercury | Iodine and Hypo |
| Methods to remove picric acid color | 50% alcohol and 70% alcohol (longer) |
| Function of dehydration with clearing | Prepares sections for mounting and removes H2O |
| Funtion of 95% alcohol after eosin staining | Differentiate and dehydrate |
| When slides are not destained or differentiated the cytoplasm appears | Hazy with poor nuclei contrast |
| When tissues are incompletely dehydrated the appear: | Opaque |
| When clearing xylene are contaminated, they appear: | Milky white |
| Washing the tissue in water after fixation prevents | Chrome artifact |
| Zenker's & Helly's | May form chrome pigment |
| Placing tissue in seneral changes of 50% alcohol removes | Picric acid color |
| A dark brown crystalline birefringence artifact is caused by | Formalin at pH 5.6 |
| Volumes of fixative used for every volume of tissue | 15-20 |
