| A | B |
|---|
| Ocular | Eyepiece |
| Microscope used to study living material | Phase contrast |
| 2 separate magnifying lenses of the microscope | Ocular & Objectives |
| Maximum magnification of a light microscope | 1000x |
| Total magnification for examining your knife edge under the microscope | 100x |
| To find the total magnification of a light microscope | Multiply the ocular (10x) by the highest power objective (100x) |
| 3 components of the lens system of a compound microscope | Condenser/Objectives/Ocular |
| Functions of the condenser | Concentrates light on tissue sections |
| MSDS are obtained from | The Manufacturer |
| Storage temperature for Glacial acetic acid | 25°C |
| Formaldehyde’s PEL | 0.75 ppm |
| Formaldehyde’s STEL | 2 ppm |
| Highest number on the NFPA diamond | 4 |
| Blue on the NFPD diamond represents | Health |
| Red on the NFPD diamond represents | Fire |
| Disposable blades should be discarded in | Plastic sharps container |
| Class A fires involve this type of materials | Paper, textiles, wood |
| Substance in a histology lab that is both radioactive and an oxidizer | Uranyl Nitrate |
| Type of extinguisher used if the pH meter catches on fire | Class C - electrical |
| Carcinogenic substances | Schiff’s, Formalin, Benzene |
| 2.7 gms of Hematoxylin diluted to 100 ml is what percent solution? | 2.7% |
| 1 gm giemsa powder diluted to 100 ml is what percent solution? | 1% |
| 2.5 ml of eosin diluted to 500 ml equals what percent solution? | 0.5% |
| Prepare 25 ml of 20% ethyl alcohol from 95% ethyl alcohol | 5.26 ml of alc + 19.74 ml H2O |
| Convert 60 degrees F to C | 15.5°C |
| Convert 38 degrees C to F | 100.4°F |
| Convert 95 degrees F to C | 35°C |
| Convert 40 degrees C to F | 104°F |
| A micron = | 0.001 of a mm |
| Knife used in Electron Microscopy for THIN sectioning | Diamond |
| Knife used in Electron Microscopy for THICK sectioning | Glass |
| Problems associated with fixed tissue for frozen sections | Formation of ice crystals & Section does not adhere to slide |
| Cryostat temperature for Brain | -7 to -10°C |
| Cryostat temperature for Breast | -13 to -30°C |
| Cryostat temperature for Muscle & LN | -13 to -20°C |
| Sections collect on knife-edge during Frozen Sectioning | Temp too warm/Anti-roll plate incorrectly positioned/Rough leading edge on anti-roll plate |
| Sections go over anti-roll plate during Frozen Sectioning | Anti-roll plate incorrectly positioned |
| Artifactual holes noted during Frozen Sectioning | Immerse tissue in liquid nitrogen |
| Cryostat is stiff and difficult to operate | Lubricate microtome |
| Proper storage of a microtome knife | Oil to store/Before using remove oil with Xylene then absolute alcohol |
| To correct Thick – Thin Sections | Tighten knife & block |
| Over dehydrated tissue from the tissue processor | Tissue becomes very hard or brittle |
