| A | B |
|---|
| The microscope used to study living material (cell cytoplasm, nucleus and inclusions) | Phase contrast |
| Magnifying lenses in the compound microscope | Ocular & Objectives |
| Maximum magnification of light microscopes | 1000x |
| Calculation for the total magnification of a light microscope | 10 x 100 = 1000x |
| Condenser, Objectives, Ocular | Components of the lens system o fa compound microscope |
| The functions of the condenser: | Converges light giving full resolving power of objectives |
| To obtain a MSDS you would need to contact: | Manufacturer |
| Glacial acetic acid should be stored at | 20 to 25 Degrees Celsius |
| Formaldehyde's PEL = | 0.75 ppm |
| The highest number that may be used for the hazard rating | 4 |
| Disposable blades should be discarded in: | Plastic sharps container |
| Class A fires involve | Paper, textiles, wood |
| Substance in a histology lab that is slightly radioactive | Uranyl Nitrate |
| If the pH meter catches on fire which type of extinguisher would you use? | Class C - electrical |
| Carcinogenic substances which may be found in a histology lab: | Schiff's, Formalin, Benzene |
| A micron | 0.001 of a mm or 1/1000 of a mm |
| Knife used in EM when sectioning the hardest materials | Diamond |
| Problem when sectioning frozens that have been fixed | Ice crystals and sections fall off the slide |
| Temperature of cryostat for the brain | -7 to -10 Degrees Celsius |
| Temperature of cryostat for the breast | -13 to -30 Degrees Celsius |
| Temperature of cryostat for the lymph node | -13 to -20 Degrees Celsius |
| Frozen sections collect on knife-edge | Temp too warm or anti-roll plate incorrectly positioned |
| Frozen sections go over anti-roll plate | Anti-role plate incorrectly positioned |
| Artifactual holes noted in frozen sections | Immerse tissue in liquid nitrogen |
| Cryostat is stiff and difficult to operate | Lubricate microtome |
| Proper storage of a microtome knife | Oil before putting away |
| Problem Solving Thick-Thin Selections | Make sure knife and block are tight |
| If the tissue is over dehydrated | Tissue becomes very hard or brittle |
