| A | B |
|---|
| Preserves minute calcium deposits | Hollande |
| Fixative of choice for Giemsa stains | Zenker's |
| Alcian blue stains these positive | Goblet cells |
| Overfixation with formalin may cause | Masked antigenic sites |
| Need a reducing agent for silver stains | Argyrophilic |
| Disolves urates | Water |
| Fixative that penetrates tissue rapidly | Carnoy's |
| Diamine-silver complex is very | Alkaline |
| When using toluidine blue | Coverslip from water |
| A nick in the knife edge | Knife line |
| Formalin buffer | Sodium phosphate |
| Dye ini Schiff's reagent | Basic fushsin |
| Leaving tissue too long in the clearing step on the processor | Hardens and shrinks the tissue |
| Feulegen's & PAS demonstrate | Adehyde groups |
| If you get poor results using the Giemsa stain | Adjust the pH |
| Requires treatment with iodine and hypo | Zenkers and Helly |
| Turnbull blue demonstrates | Ferrous ions |
| Glutaraldehyde | Cross-links proteins |
| Weigert hematoxylin | Resistant to decolorization with acidic stains |
| The tissue in a paraffin block crumbles and seems brittle | Due to prolonged dehydration |
| Excess picric acid is removed by | 70% alchohol |
| A dye lake is formed with a | Mordant |
| Eosin gives | 3 shades of pink |
| Uranyl nitrate's box is wet and damaged | Have the radiation safety officer check the package for radiation leaks |
| When chromic acid is dark brown | It is reduced |
| Sectioning with static electricity | Breathe on the section |
| Formalin pigment is removed with | Potassium hydroxide in 80% alcohol |
| OCT | Embedding media for FS |
| Aqueous mountants | Subject to air bubbles |
| Synthetic mountants | Good when tissue transparency is important |
| Crystal violet substance staining rose colored | Amyloid |
| Crooked paraffin ribbons | Realign the block |
| A fixative that preserves all bone marrow elements including red blook cells | Helly |
| Blue staining of muscle and red staining of collagen | Mallory PTAH |
| A long shelf-life for hematoxylin has | A resevoir of unoxidized hematoxylin |
| Fixative stored at room temperature | Zamboni's |
| Zinc sulfate | Improves the quality of nuclear fixation |
| If tissue is not laying flat on the water bath | Lay the section on 10% alcohol, then transfer to the flotation bath |
| Ethyl alcohol fixation causes | Excessive shrinking of tissue |
| Gomori aldehyde fuchsin stains | Purple |
| Alcohol on the tissue processor | Removes water |
| Label hazardous waste containers with | Chemical names, concentration, and date |
| Brown-Brenn Gram stains gram-negative bacilli with | Basic fuchsin |
| To prevent holes in the tissue with frozen sections use | Liquid nitrogen |
| Basement membranes demonstrates with | Periodic acid-Schiff |
| When embedding paraffin is 75°C it may cause | Cracking of the blocks |
| If the nuclei are over-stained with a H&E | Differentiate the slides |
| Bacteria stain differentially with the Gram stain due to the | Cell wall |
| Fixative of choice for uric acid crystals | Absolute alcohol |
| Reticulin staining method | Diamine silver nitrate |
| Processing of delicate tissue begins with | 50% alcohol |
| 10cm3 equals | 10ml |
| Copper stain | Rhodanine |
| Alizarin red S | Demonstrates Calcium |
| Digestion substance for gycogen | Amylase |
| The alkaline nature of silver solution | May cause the tissue to fall of the slide |
| Carboxylated acid mucosubstance | Positive with AB pH 2.5 and negative with pH 1.0 |
| Uses a fluorochrome dye | Thioflavin T |
| To prevent the formation of a thick layer of paraffin on the bottom of the mold | Embed quickly and neatly |
| Wet tissue should be stored in | 70% alcohol |
| Melanin is bleached with | Potassium permanganate |
| Neutral polysaccharide | Positive with PAS, negative with AB |
| Benzene | Carcinogenic |
| Cresyl echt violet | Demonstrates basophilic material in neuron cell bodies |
| Cut sections at two micrometers to study | Basement membranes |
| Neutral buffered formalin is good for | EM specimens |
| Mallory phosphotungstic acid hematoxylin demonstrates | Fibrin |
| Bone sections left in decal solution too long | Reveals little or no nuclear detail |
| Use hyaluronidase with alcian blue for | Connective tissue mucin |
| Deficiency of iron storage is demonstrated with | Prussian blue |
| Cryostate knives are made of | A non-corrosive alloy |
| Schiff solution | Leuco reagent |
