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Genetic Engineering

Terms and concepts used to describe techniques of gene technology.

AB
plasmidsmall, circular DNA found and transfered between bacteria
recombinationwhen genetic material from two different bacteria are combined into a single genome
conjugationbacterial sex using a pili
transformationnecrophilic form of bacterial sex; the uptake of DNA by bacteria through their cells walls
lyticdescribes a virus that lyses its host
retrovirususes reverse transcriptase to copy DNA from RNA
temperatedescribes a virus that may incorporate itself into a bacteria and remain dormant
provirusan integrated virus that is dormant
transductionwhen a virus carries genes from one bacterial cell to another
transposons"jumping" genetic elements
L1 Retrotransposon5% of the human genome is composed of this mobile genetic element
sticky endsplaces where DNA is cut by restriction enzymes
restriction enzymesused by bacteria to chop up unwanted viral DNA
cloningproducing duplicate copies of a genome
screeningmethod for locating colonies with recombinant plasmids
PCRa method for amplifying DNA from small samples
insulinfirst genetic engineering "product"
probea radioactive substance used to find a particular section of DNA
DNA sequencingdecoding DNA into its message of 4 bases
DNA fingerprintingidentifying an individual using their unique DNA banding pattern
librarya collection of transformed bacteria that each contain different pieces of recombinant DNA
Barbara McClintockdiscovered transposons in maize
Max Delbruckdiscovered the lytic cycle of viruses
pilusa cytoplasmic extension
influenzaexample of a disease caused by transduction of a virus
diphtheriaexample of a bacterial disease worsened by infection by a phage carrying toxin genes
antibiotic resistancecarried on plasmid
cDNAmade from retroviral RNA


Instructor
Bandera High School
Bandera, TX

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