| A | B |
|---|
| Magnification | = product of the magnification of ocular and objective lens |
| Resolution expressed | as “D” minimum distance between two objects |
| Fluorchrome | = molecule that absorbs light a specific λ and emits light at another λ |
| Direct fluorescent microscopy: | fluorochrome binds directly to target molecules in specimen |
| Indirect fluorescent microscopy: | mAb binds to target (1º) and a second antibody conjugated to a fluorochrome (2º) binds to the 1º |
| Confocal Microscopy | Specimens are “stained” with fluorochromes and Light source is laser beam and Light emitted from excited fluor comes from focal plane |
| Electron Microscopy | Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) |
| Scanning electron microscopy (SEM) | Specimens coated with metal |
| Transmission electron microscopy (TEM) | Specimens usually fixed and embedded in epoxy resin, thin sections, prepared using ultramicrotome, are stained with heavy metals |
| Fractionation of Cells | Differential centrifugation and Density gradient centrifugation |
