| A | B |
|---|
| Yeast | Early studies done with secretion mutants |
| Secretory Pathway | Synthesis and translocation across RER membrane |
| Living cells are “pulsed” with | radio-labeled amino acids |
| Living cells are washed | to removed labeled amino acids |
| Secreted Proteins | Leave ER in transport vesicles through Golgi |
| Integral Membrane Proteins | Plasma membrane and Vesicle membrane |
| Topology of Membrane Proteins | Number of times the polypeptide chain of a membrane protein crosses the membrane |
| N-linked glycosylation initiated in RER | and completed in Golgi complex and O-linked glycosylation in Golgi complex |
| N-linked Glycosylation in RER | 2 N-acetyl glucosamine residues, 9 mannose residues, 3 glucose residues |
| Functions of Glycosylation | Folding, Stability, and Cell:cell interaction |
| Disulfide Bond Formation | Occur between sulfhydryl groups (SH) on cysteine residues |
| Folding and Assembly of Proteins in ER | Chaperones and Formation of disulfide bonding |
