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Cell Bio 4100 Intracelluar Tracking of Proteins Part 2

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Clathrin-coated Vesicles“Double-coated” vesicles and Clathrin molecule
Clathrin molecule—triskelion3 heavy chains + 3 light chains
AP ComplexesAP1, AP3 coated vesicles, AP2 coated vesicles
DynaminNecessary for “pinching off” vesicle
Constitutive secretion—continuous secretion
Regulated secretiondepends on cell signaling events
Aggregation of cargo appears to take place intrans-Golgi
Initial assembly of membrane proteins appearsto occur in trans-Golgi
Both apical and basolateral proteins go tobasolateral aspect of cell
Apical proteins move to apical aspect of cell bytranscytosis


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