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Biotech 2.2 Genetic Transformation

AB
AmpicillinAntibiotic related to penicillin that inhibits the synthesis of peptidoglycan
Antibiotic– Chemical that prevents or reduces the growth of microorganisms
ArabinoseA sugar from plant gums, used as a culture medium in bacteriology.
Bioluminescence– The production of light by means of a chemical reaction in an
CentrifugationSpinning a mixture at a very high speed to separate heavy and light
ChromatographyA process for separating complex liquid mixtures of proteins or
Competent cell– Cell with the ability to take up extracellular DNA from the
ElectroporationTransformation of cells using an electrical field to increase
Elution bufferThe buffer used to detach a protein or nucleic acid from
Lysozyme– Enzyme needed to lyse, or break open bacteria cell walls. The enzyme
PelletIn centrifugation, the heavier particles such as bacteria or cellular membranes
PlagiarismAn act or instance of using or closely imitating the language and thoughts
PlasmidDNA molecule (usually circular) a few thousand base pairs in length capable
PurificationThe process of eliminating impurities from a sample
ResearchDiligent and systematic inquiry or investigation into a subject in order to
SupernatantLiquid portion of a centrifuged sample (the solid portion on the bottom of
TransformationUptake of DNA from the outside environment, usually by bacteria;
TransgenicA transgenic organism is one that has been altered to contain a gene
UV (ultra violet) lighta wavelength of light that is used to detect colorless
Green Fluorescent Proteina genetic trait isolated from a jelly fish


Agriscience teacher
South Carroll High School
MD

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