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Gram Stain Part 1

AB
What stain is the mordant stain?Grams Iodine
What stain is the counter stain?Safranin
What stain is considered the primary stain?Crystal Violet
What stain is considered the decolorizer?Alcohol/Acetone
What two stains combined form a complex unable to escape the cell wall of gram positive bacteria?Iodine and Crystal Violet
What color are gram positive bacteria after the entire gram staining process?Purple
What color are gram negative bacteria after the entire gram staining process?Red/Pink
After the alcohol/acetone stain is applied to a gram positive organism, the cells will appear what color?Purple
After the alcohol/acetone stain is applied to a gram negative organism, the cells will appear what color?Clear
After the mordant stain is applied to a gram positive organism, the cells will appear what color?Purple
After the mordant stain is applied to a gram negative organism, the cells will appear what color?Purple
After the counter stain stain is applied to a gram positive organism, the cells will appear what color?Purple
After the counter stain stain is applied to a gram negative organism, the cells will appear what color?Red/Pink
Which cells have a thicker cell wall?Gram Positive
Which cells have an outer membrane?Gram Negative
In what type of cell is the cell wall decreased to the point that the crystal violet/iodine complex can not escape?Gram Positive
What stain is applied for 2 minutes rather than 1 minute?Safranin
What stain is applied 2nd?Grams Iodine
What stain is applied 3rd?Alcohol/Acetone



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