A | B |
ink, paints and foods | contain mixtures of coloured compounds |
chromatogram | paper with separated components |
The solvent | is the mobile phase |
The paper | contains the stationary phase |
Rf value = | distance moved by spot / distance moved by the solvent |
Rf values are always | less than 1 |
If pink spots move 4cm and the solvent has moved 10cm | the Rf value will be 0.4 |
If blue spots move 5cm and the solvent has moved 10cm | the Rf value will be 0.5 |
The starting positions of the samples | should always be above the solvent line |
the sample line | should be drawn in pencil |