A | B |
dry heat, autoclave, chemical vapor | Three methods of sterilization |
sterilization | a process that kills microorganisims |
biological indicators | vials,or strips, also known as spore tests, that contain harmless bacterial spores and are used to determine whether a sterilizer is working |
clean area | side where sterilized instruments,are prepared and stored |
contaminated area | side where contaminated items are brought for precleaning |
critical, non-critical, semi-critical instruments | instruments classifications used to determine the method of sterilization |
critical instuments | items that are used to penetrate soft tissue and bone need a high level of biocidal activity |
non-critical instruments | instruments that come in contact with intact skin only low level of biocidal activity |
semi-critical instruments | instruments that come in contct with the oral tissue and do not penetrate the skin has a medium level of biocidal activity |
ultrasonic cleaner | used to pre clean instruments |
autoclave | means of sterilizing instruments by moist heat under pressure |
dry heat sterilization | must be 170 degrees for one hour (60 minutes) |
autoclave sterilization | must be 121 degrees under pressure for twenty minutes (20 minutes) |
chemical sterilization | must be 127 degrees under pressure for twenty minutes(20) |
autoclave packaging | pack loosely to permit heat to reach all instruments |
unwrapped sterilized instrumentss | must be used immediately |
sound waves | this is how the ultrasonic cleaner works |
instruments are packaged | to maintain sterility |
liquid sterilant 2-3.4 % glutaraldehyde | must be used to sterilize plastics which are damaged by heat sterilization. Sterilization in glutaraldehyde requires 10-hours |
package instruments loosely | to enable steam to penetrate the package |
glutaraldehyde | is classified a high level disinfectant sterilant |
disadvantage of using chemical vapors to sterilize | adequate ventilation is essential because formaldehyde and methyl alcohol is released when the door is open |
ethylene oxide gas | used on plastic and rubber items that would melt in heat sterilizers |
chemical sterilant for instruments | used to sterilize if etheylene oxide is absent |
60 minutes | is the approximately time it will take to sterilize in a dry heat autoclave |
sterilization room | contaminated area (dirty side) and non-contaminated side(clean side) should be separated |
microorganism used to test for sterilization | steam autoclave, Bacillus stearothermophilus |
microoraganism used to test for sterilization | dry heat, Bacillus subtilis |
microorganism used to test for sterilization | chemical vapor, Bacillus stearothermophilus |
microorganism used to test for sterilization | Ethylene oxide, Bacillus subtilis |