| A | B |
|---|
| Nucleolus | ribosome factory, not membrane bound (***) |
| Pol I makes | rRNA one large ( Pre-rRNA) |
| Pre-rRNA cleaved to | 28s, 18s,5.8s |
| ribosomes are composed of | RNA and protein |
| what is transcribed by Pol III? | 4 different rRNAs |
| Nuclear Transport | takes mRNA, tRNA, and ribosomes from nucleus to cytoplasm |
| Transfer RNA | Clover Leaf conformation due to internal double helices |
| tRNA form | L shape |
| Nuclear pore complexes made of | nucleoporins |
| Anticodon | nucleotide triplet complementary to specific codon of mRNA |
| Anticodon | binds a codon to specify an amino acid |
| tRNA charging | reach the cytoplasm, must be bound to their specific amino acids |
| aminoacyl-tRNA synthease | binds 3OH' of the tRNA with the carboxyl group of it AA. requires energy from ATP hydrolysis |
| Triplet | Every three bases of the mRNA sequence, read 5'-3' |
| Collinear | Codon sequence is parallel to the amino acid sequence |
| Non-overlapping | next codon begins where last one ends |
| Unambiguous | ? |
| Degenerate | ? |
| Wobble | ? |
| Universal | ? |
| Start Codon | AUG |
| Translation Pathway | initiation, elongation, release |
| Initiation | Ribosomes assembled with mRNA sandwich between large and small subunits along with first charged tRNA. Binds to P site of ribosome |
| Elongation | Addition of amino acid to growing Polypeptide chain |
| Release | All come apart when it reaches stop codon |
| stop codon | UAA,UAG,UGA |
| A site | region of ribosome that binds charged tRNA for the next amino acid to be added to growing polypeptide |
| P site | region of ribosome that binds tRNA that is attached to growing polypeptide |
