| A | B |
|---|
| Describe Autolysis | Digestion of tissues by enzymes |
| The effect of lower temps on fixation include | Delays fixation time and slows diffusion and autolysis |
| Major intents of fixation | Preserves microanatomical structures and stops autolysis and putrefaction |
| What is the volume of fixative that should be used for each volume of tissue? | 10-20 |
| What osmolality of solution is best for fixation? | Hypertonic |
| Most vital step in production of a quality tissue slide? | Fixation |
| Functions of a good fixative include: | Rapid penetration, kills and hardens, allows tissues to be susceptible to staining, preserves tissue structure |
| Decay of tissue by bacteria is called: | Putrefaction |
| Most widely used fixative | Formaldehyde |
| How is 10% formalin prepared? | By mixing a 1 to 9 ratio of stock formaldehyde and water |
| Describe Glutaraldehyde | Noncoagulant, additive fixative; Is fixative of choice for fine structure |
| Describe Mercuric Chloride | Coagulant, additive fixative; Soluble in water and alcohol – is the most common coagulant fixative used in fixative mixtures |
| Describe Acetic Acid | Non-coagulant, non-additive fixative Most commonly used acid in fixative mixtures, rarely used alone in fixation |
| Describe Glyoxal | Non-coagulant, smallest dialdehyde, lyses red blood cells |
